Her first appearance from "Nene's Interactive Suicide".
Nene is a character made by Tom Fulp, who first appeared in Nene's Interactive Suicide in 1999. She is a young Japanese girl who is known for her sexual activities: her suicide was prompted by an outbreak of herpes . In Pico's School, she begs Pico to end her life, which the player may or may not do. In other movies, she is shown wielding knives to fight enemies, especially in House of 1000 Cats and Pico vs. Convict. She is also featured in a number of other games and movies, including Newgrounds Rumble, Pico Sim Date, and Pico vs. Uberkids.
As an image, Nene appears here and there on the Newgrounds website, like other Pico series characters.